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. Author manuscript; available in PMC: 2009 Dec 9.
Published in final edited form as: Biochemistry. 2009 Jun 9;48(22):4871–4880. doi: 10.1021/bi900338n

Figure 4.

Figure 4

Effect of phosphate analogues on the digestion pattern of T. maritima CopA. SDS–PAGE was performed as described in Experimental Procedures. Purified CopA (0.5 mg/mL) was incubated with 0.5 mg/mL papain at 50 °C for 30 min in the presence of 1 mM BCS (no copper added), in the absence or presence of 2 mM ATP, or following preincubation with 0.2 mM MgF, 0.2 mM AlF, 0.2 mM BeF, or 0.2 mM Be(NO3)2.