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Effect of copper on formation and subsequent processing of the phosphoenzyme upon addition of ATP to CopA in membrane vesicles obtained directly from expression host cells. The reaction was started by the addition of 25 μM [γ32P]ATP to membrane vesicles including T. maritima CopA (25 μg/mL) in the presence of 1 mM BCS (a) or 25 μM CuCl2 (b) at 60 °C (see Experimental Procedures for details).
