Table 1. In vitro culture of 17.5 dpc mouse fetal ovaries under various E2 concentrations in the presence or absence of FSH (n=4 ovaries for each group).
Group | 1st three days culture | 2nd three days culture |
---|---|---|
1 | BM | BM |
2 | BM +10-6 M E2 | BM +10-6 M E2 |
3 | BM +10-10 M E2 | BM +10-10 M E2 |
4 | BM +10 mIU/ml FSH | BM +10 mIU/ml FSH |
5 | BM +10-6 M E2+10 mIU/ml FSH | BM +10-6 M E2+10 mIU/ml FSH |
6 | BM +10-10 M E2+10 mIU/ml FSH | BM +10-10 M E2+10 mIU/ml FSH |
7 | BM +10-6 M E2 | BM +10-6 M E2+10 mIU/ml FSH |
8 | BM +10-6 M E2 | BM +10-10 M E2 |
9 | BM +10-6 M E2 | BM +10-10 M E2+10 mIU/ml FSH |
10 | BM +10-6 M E2 | N/A |
11 | BM +10-6 M E2+ 10 mIU/ml FSH | N/A |
12 | BM +10-10 M E2 | N/A |
13 | BM +10-10 M E2+10 mIU/ml FSH | N/A |
BM: basal medium.