Skip to main content
. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: J Dent Res. 2009 Apr;88(4):323–327. doi: 10.1177/0022034509334240

Figure 3. DPPI Activates Pro-KLK4 In Vitro.

Figure 3

A) Hydrolysis of the KLK4 specific substrate (Boc-Val-Pro-Arg-AMC) was measured by monitoring fluorescence every 1 min over 2 hours with 380 nm excitation and 460 nm emission wavelengths. The fluorescent substrate was incubated with thermolysin, DPPI, pro-KLK4, thermolysin-activated KLK4 (th-activated KLK4) and DPPI-activated KLK4. B) Fluorescence measurement with the same enzymes after 2 hour incubation of the Boc-Val-Pro-Arg-AMC substrate (n=4). Incubation with thermolysin, DPPI or pro-KLK4 does not result in substrate cleavage. Thermolysin-activated KLK4 (th-activated KLK4) and DPPI-activated KLK4 both cleaved the substrate as indicated by the fluorescence. Data are presented as mean ± SEM.