FIG. 3.

Rostral-caudal extent of 3-NT staining at all time-points as shown by immunohistochemistry. All sections were counterstained with nuclear fast red. Images were taken every 2 mm starting 6 mm rostral to the epicenter, and ending 6 mm caudal to the epicenter. At each time-point, the images were taken from only one animal. By 3 h post-injury, staining was elevated above sham levels at the epicenter and for several millimeters in both the rostral and caudal directions. The largest spread of staining was present between 24 and 72 h post-injury, with 3-NT apparent at least 6 mm from the epicenter in either direction. By 1 week post-injury, staining had largely abated and was found sparingly and only in sections within 2 mm of the epicenter. At 2 weeks post-injury staining had returned to near-sham levels even at the epicenter. (Compare Figure 3 with Figure 2, which shows lipid peroxidative-related 4-HNE staining of the adjacent sections [scale bar =500 μm]).