FIG. 5.

Higher-power views of 3-NT oxidative damage in the injured spinal cord. The top row shows a 24-h post-injury spinal cross-section at 1 mm caudal to the epicenter, and shows intense oxidative damage staining of gray matter microvessels (arrows), as well as surrounding parenchymal elements. The middle row displays a 72-h post-injury cross-section 1 mm rostral to the epicenter, which again shows an example of microvascular staining (arrow). The bottom row shows an example at 24 h that was taken 3 mm caudal to the epicenter, in which intense 3-NT staining of presumptive dorsal commissural axons and cell bodies is apparent (4× scale bar =500 μm; 20× scale bar =200 μm; 40× scale bar =100 μm).