PEITC reduces the steady state levels of ER-α36 protein. MCF7 (A) and H3396 (B) cells were treated with vehicle (DMSO), 1, 2.5, 5, and 10µM of PEITC or ICI 182780 for 12 hours and lysed for Western blot analysis with anti-ER-α36 and anti-β-actin antibodies. MCF7 (C) and H3396 (D) cells were treated with 5µM of PEITC or ICI 182, 780 for indicated time points, and lysed for Western blot analysis with anti-ER-α36 and anti-β-actin antibodies. Experiments were repeated three times, and the representative results are shown.