Timeline for effective melanocyte ablation. Fish were treated for 2-day periods with either 5 µM NCP or 10 µg/mL MoTP at 1, 3, 5, 7, 9, 11, 13, and 15 dpf and assessed for melanocyte ablation. Fish were treated for 7-day periods with 20 µg/mL/MoTP at >180 dpf or 750nM NCP at 21, 28, 35 dpf, and >180 dpf. The effectiveness of the laser protocol represents a variety of experiments on larval1 and adult (O’Reilly-Pol, unpublished data) fish. Effective periods for each method are designated by the bar under the timeline (black for MoTP, shaded for NCP, and open for laser).