(A-C) Midsagittal sections of BrdU (red) and DAPI (blue) stained wild-type (A), Lmx1a−/− (B), and Lmx1a−/−; Lmx1bcko/− (C) cerebella anlage at E12.5. Arrowheads show BrdU staining in the ventricular zone. Proliferation was reduced in Lmx1a−/− and Lmx1a−/−; Lmx1bcko/− embryos.
(D) Mitotic index of E12.5 ventricular zone (BrdU/DAPI). Lmx1a−/−; Lmx1bcko/− embryos showed significant reduction compared to Lmx1a−/− embryos, which was reduced compared to wild-type embryos. * indicates p<0.01; ** indicates p<0.001. Bars indicate standard error.