Panel A. Gas-chromatography of ascorbic acid 2, 3, 5, 6,-tetrakis-O-(trimethylsilyl) ether derivative and its nitro adduct. Ascorbic acid derivative (M 464) was formed as addition of TMS4 (288) to ascorbic acid MW (176). Nitro adduct (M+31) is represented by small peak (M 495). Panel B. Mass spectrum of ascorbic acid (molecular ion with methyl group loss (M-15), M/z = 449. Panel C. Mass spectrum of Ascorbic acid Nitro adduct (molecular ion (M+35) M/z = 495).