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PCR primers and conditions
| Gene | mechanism | NCBI accession number | PCR conditions |
Primers (5′-3′) | |
|---|---|---|---|---|---|
| Annealing (ºC) | cycles | ||||
| β-actin | cytoskeleton | AF057040 | 58 | 28 | F: TGCCATGTATGTGGCCATCCA |
| R: ACCTCCAGACAGCACTGTGT | |||||
| Caspase-3a | apoptosis | NM131877 | 57 | 31 | F: TGTGTTGCTCAGTCACGGC |
| R: GGCATGTTGGAGGTGGACTC | |||||
| DDB2 | DNA repair (NER) | NM001083061 | 57 | 38 | F:AAAAGACCGAATGAAGAAACTCC |
| R:TAGTAAGCAACTTGGTGCTGTCA | |||||
| Ku80 | DNA repair (NHEJ) | AY877316 | 57 | 31 | F:TGGAGGAGATTGAGAGAGAACTG |
| R:GTTTCATCATCATCGTTCAGACA | |||||
