Coomassie blue-stained sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gel of plant samples, 60 days after transplanting. Two independent lines (1, 2) for each construction were analyzed. Recombinant proteins GFP-2L21 and CTB-2L21 are marked. Fifty micrograms of plant total protein was loaded per well. 2L21 epitope from the VP2 protein of the canine parvovirus, CTB cholera toxin B, GFP green fluorescent protein, MW molecular weight marker, WT wild-type Petit Havana plant