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. Author manuscript; available in PMC: 2009 Oct 20.
Published in final edited form as: Curr Top Microbiol Immunol. 2009;332:33–54. doi: 10.1007/978-3-540-70868-1_3

Fig. 8.

Fig. 8

Titers of antibodies at day 50 induced by plant-derived CTB-2L21 recombinant protein. Balb/c mice were intraperitoneally immunized with leaf extract from CTB-2L21 transgenic plants. Animals were boosted at days 21 and 35. Each mouse received 20 μg of CTB-2L21 recombinant protein. Individual mice sera were titrated against 2L21 synthetic peptide, VP2 protein and control peptide (amino acids 122–135 of hepatitis B virus surface antigen). Titers were expressed as the highest serum dilution to yield twice the absorbance mean of preimmune sera. M1—M6 mouse 1–6, 2L21 epitope from the VP2 protein of the canine parvovirus, CTB cholera toxin B, VP2 protein of the canine parvovirus that includes the 2L21 epitope