. Author manuscript; available in PMC: 2009 Oct 20.

Published in final edited form as: Curr Top Microbiol Immunol. 2009;332:33–54. doi: 10.1007/978-3-540-70868-1_3

Table 1.

List of vaccine antigen proteins expressed via the chloroplast genome

Vaccine antigens	Gene	Site of integration	Promoter	5′/3′ Regulatory elements	% TSP expression	Functionality assay	Lab/year
Cholera toxin	CtxB	TrnI/trnA	Prrn	ggagg/ TpsbA	4%	GM—1 ganglioside binding assay	Daniell 2001
Tetanus toxin	TetC (bacterial and synthetic)	TrnV/rps12/7	Prrn	T7 gene 10a, atpB^b / TrbcL	25% ^a, 10% ^b	Positive systemic immune response	Maliga 2003
Canine parvovirus (CPV)	CTB—2L21/GFP—2L21	TrnI/trnA	Prrn	PpsbA/TpsbA	31.1%, 22.6%	Immune response	Daniell, Veramandi 2004
Anthrax protective antigen	Pag	TrnI/trnA	Prrn	PpsbA/TpsbA	4%–5%	Macrophage lysis assay	Daniell 2004
Plague vaccine	CaF1~LcrV	TrnI/trnA	Prrn	PpsbA/TpsbA	14.8%	ND	Daniell 2003