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. Author manuscript; available in PMC: 2019 May 14.
Published in final edited form as: Nature. 2018 Nov 14;563(7732):501–507. doi: 10.1038/s41586-018-0692-z

Figure 3 |. Application of AaegL5 to resolve the sex-determining locus and the HOX gene cluster.

Figure 3 |

a, M locus structure indicating high alignment identity (grey dashed boxes) and boundaries of myo-sex and Nix gene models (magenta and white boxes, arrowhead represents orientation). b, FISH of BAC clones containing myo-sex and Nix. Scale bar: 2 μm Representative image of 10 samples. c, De novo optical map spanning the M locus and bridging the estimated 163 kb gap in the AaegL5 assembly. DNA molecules are cropped at edges for clarity. d, Chromosome-quotient (CQ) analysis of genomic DNA from pure male and female libraries aligned to AaegL5 chromosome 1. Each dot represents the CQ value of a non-repeat-masked 1 kb window with >20 reads aligned from male libraries.