Skip to main content
. Author manuscript; available in PMC: 2019 Jul 29.
Published in final edited form as: Cell Chem Biol. 2019 Feb 7;26(4):584–592.e6. doi: 10.1016/j.chembiol.2019.01.003

Figure 1.

Figure 1

Comparison of Golgi labeling with Halo- and SNAP fusion proteins of sialyl transferase. A) Top: Scheme of the labeling procedure; Bottom: Confocal images of live HeLa cells that have been treated as described in the scheme above. The white arrows indicate cells that express ManII-GFP and have been labeled with SiR-CA or SiR-BG. Scale bar: 20 μm. B) Quantification of cells expressing ManII-GFP that are positive for SiR from three independent experiments (ST-Halo: 740 cells in total; ST-SNAP: 837 cells in total). C) Fluorescence intensity distribution of HeLa cells that were incubated with SiR-CA or SiR-BG and that are transiently expressing ST-Halo-HA, ST-SNAP-HA or no fusion protein. The number of cells (n) analyzed is indicated in the plot.