Skip to main content
. Author manuscript; available in PMC: 2019 Aug 9.
Published in final edited form as: J Clin Transl Sci. 2019 May 14;2(6):350–355. doi: 10.1017/cts.2019.3

Figure 2.

Figure 2

Functional consequences of rs146534657:A>G and rs2230926:T>G. a Wild-type A20 plasmid or plasmids containing the rs146534657:A>G (N102S) and rs2230926:T>G (F127C) were transfected into A20 knockout 293 cells at the indicated concentrations along with an NF-kB firefly luciferase reporter. Cells were stimulated with 10ng/ml of TNFα (TNF) for 8 hours then harvested for luciferase assay. Firefly luciferase activity was normalized to a co-transfected Renilla luciferase reporter. Total plasmid concentrations were normalized using empty pCMV vector, b Protein from these cells were also used in western blot. Quantifications of transfected A20 band intensities shown as immuno-blots (IB) are normalized to the housekeeping protein GAPDH. ** P < 0.01. Figures are representative of three independent experiments.