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. Author manuscript; available in PMC: 2020 Mar 25.
Published in final edited form as: J Thorac Cardiovasc Surg. 2019 Feb 12;157(5):2096–2106. doi: 10.1016/j.jtcvs.2018.12.105

Figure 8:

Figure 8:

Exosomes isolated from LTxRs with bronchiolitis obliterans syndrome (BOS) contain lung SAgs Col-V and Kα1T. Exosomes were isolated from plasma of LTxRs with BOS (n=10) and from stable LTxRs (n=10) and were subjected to immunoblot. A) Western blot demonstrates that exosomes from LTxRs with BOS contain lung SAgs Col-V and Kα1T, but exosomes from stable LTxRs do not. B) Densitometry analysis shows significant increase in Col-V (p=0.043) and Kα1T (p=0.0173) in exosomes from LTxRs with BOS but not in exosomes from stable LTxRs. CD9 was used as loading control marker. Box-whisker graph signified median of each group with lower to higher range: Col-V: BOS median: 1.511 fold change (interquartile range, 0.962-2.111 fold change), stable median, 0.765 fold change (interquartile range, 0.628-0.953 fold change); Kα1T: BOS median 1.507 fold change (interquartile range 0.543-2.497 fold change), stable median 0.557 fold change (interquartile range 0.429-0.716 fold change). Experiment carried out in duplicate. B: bronchiolitis obliterans syndrome (BOS); S: Stable.