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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Cell Mol Life Sci. 2019 Mar 27;76(17):3449–3464. doi: 10.1007/s00018-019-03081-w

Figure 1.

Figure 1.

Short term VC treatment enhanced pepsin-resistant col1α2 secretion via a pathway independent of transcription. Time course of MEF cells (A), HFF cells (B), and HEL cells (C) treated with 50 μM of VC showed that more col 1α2 could be detected in the culture medium compared to non-treated cells. A consistent difference was first detected 3 hrs post treatment of MEF cells (A: middle panel, n=3). By 3 hrs after treatment, less intracellular col 1α2 was detected in VC-treated cells compared with non-treated cells (A: bottom panel, n=3). A consistent difference was detected 6 hrs after treatment of HFF and HEL cells (B&C: middle panel, n=3). Significantly less intracellular col 1α2 could be detected in VC treated cells compared with non-treated cells at 6 hrs after treatment (B&C: bottom panel, n=3). Laminin was used as a loading control for culture medium protein. Actin was used as a loading control for cell lysate protein. * indicates the position of col 1α2. Immunoblot densitometry was quantified using Image J and depicted graphically.