Table 3.

Primers used to amplify P4ha1 and EGFP-KDEL and mutate P4ha1.

Gene name	Sequence
P4ha1
Sense:	5’ATTTGCGGCCGCCACCATGATCTGGGTTGTTTTAATGA-3’
Antisense:	5’-CTAGCTAGCTTCCAATTCTGACAGGGTG-3’
HA-tagged-P4ha1
Sense:	5’-CGTAAAAAACCCTGGATGAGCGTAATCTGGAACATCGTATGGGTAGGCTAAAGACTGAGGAAG-3’
Antisense:	5’-CTTCCTCAGTCTTTAGCCTACCCATACGATGTTCCAGATTACGCTCATCCAGGGTTTTTTACG-3’
P4ha1-N113Q
Sense:	5’-ACTGCCTCTGAATGGTTAGTTGAGAGATGAAGCCATCCGAC-3’
Antisense:	5’-GTCGGATGGCTTCATCTCTCAACTAACCATTCAGAGGCAGT-3’
P4ha1-N259Q
Sense:	5’-GGTCGCCCGACGCTGACTTTTGGGCATCTTTTTCTTTACTCA-3’
Antisense:	5’-TGAGTAAAGAAAAAGATGCCCAAAAGTCAGCGTCGGGCGACC-3’
Signal peptide of Calreticulin
Sense:	5’-ATTTGCGGCCGCCACCATGCTCCTTTCGGTGCCG-3’
Antisense:	5’-CAGCTCCTCGCCCTTGCTCACTGCGGCGGCCAGGCCGAGGA-3’
Egfp
Sense:	5’- TCCTCGGCCTGGCCGCCGCAGTGAGCAAGGGCGAGGAGCTG-3’
Antisense:	5’- GCTCTAGATTACAGCTCATCCTTGTACAGCTCGTCCATGCC-3’