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. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: J Neurosci. 2009 Jul 29;29(30):9405–9416. doi: 10.1523/JNEUROSCI.2356-09.2009

Figure 2. Olfactory neurite outgrowth assay.

Figure 2

Olfactory epithelial explants plated on either poly-d-lysine (PDL) coated coverslips alone (A) or PDL coverslips subsequently coated with laminin (LN; as a positive control) (B), Tnc (C) or Tnr (D). Explants are grown for 48hrs then fixed and stained for NCAM and couterstained with DAPI. (A) When grown on 50μg/μl PDL modest neurite outgrowth from the explant is observed. (B) When 20μg/μl laminin is added to the substrate, neurite outgrowth is greatly potentiated, as expected. (C) When 20μg/μl Tnc is added to the substrate, neurite outgrowth is inhibited. (D) When 20μg/μl Tnr is added to the substrate, neurite outgrowth appears unaffected, as compared to PDL control explants. Scale bars = 50μm.