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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Cancer Immunol Immunother. 2019 Apr 27;68(6):999–1009. doi: 10.1007/s00262-019-02342-8

Figure 1: Peripheral immune profiling of patients with meningiomas.

Figure 1:

(a) Representative gating scheme for identification of myeloid cells from peripheral blood leukocytes by flow cytometry. Live cells were gated using forward and side scatter from the total population (left), followed by identification of single cells (left-center), gating for total myeloid population of CD45+/CD11b+ cells (right-center), and gating for PD-L1+ cells (right). (b) Summary of PD-L1 expression in monocytes from each of the 53 evaluated patients as well as healthy controls ordered by percent positive expression within each grade. Patients with grade III meningiomas had significantly elevated monocyte PD-L1 compared to patients with lower grade tumors (* p < 0.05). Patients with monocyte PD-L1 expression greater than 10% are colored in red. (c) Representative gating scheme for identification of MDSCs from peripheral blood leukocytes by flow cytometry. Monocytes were gated from the total population (left), followed by identification of single cells (not shown), gating for total myeloid population of CD33+/CD11b+ cells (center), and gating for MDSCs (HLA-Drlo; right). (d) Summary of MDSC abundance from each of the 53 evaluated patients as well as healthy controls grouped by grade. Patients with grade II and III meningiomas had increased MDSCs (* p < 0.05). (e) Representative gating scheme for identification of Tregs from peripheral blood leukocytes by flow cytometry. Live cells were gated from the total population (left), followed by identification of single cells (not shown), gating for CD4+ T-cell population (center), and gating for Tregs (CD25+, FoxP3+; right). (f) Summary of percentage of Tregs/CD4+ T-cells from each of the 53 evaluated patients as well as healthy controls grouped by grade. Treg percentage did not vary significantly between grades.