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. Author manuscript; available in PMC: 2019 Sep 12.
Published in final edited form as: J Immunol. 2019 Aug 9;203(6):1521–1531. doi: 10.4049/jimmunol.1801616

Figure 3. LPS stimulation induces c-Abl’s nuclear transportation.

Figure 3.

(a) LPS stimulation induces nuclear import of c-Abl. RAW 264.7 cells were challenged with LPS for various lengths of time. Cell lysates from nuclear and cytoplasmic fractions were subjected to western blotting to determine the sub-cellular distribution of c-Abl. (b) Immune-fluorescence staining verifies LPS-induced nuclear import of c-Abl. RAW 264.7 cells were mock-treated or LPS exposed (±STI571) for 1 h. And then cells were fixed and permeabilized, and incubated with anti c-Abl rabbit polyclonal Ab and TRITC-conjugated secondary Ab. The nuclei of the cells were stained with DAPI. Similar results were obtained from at least three independent experiments.