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. Author manuscript; available in PMC: 2019 Sep 12.
Published in final edited form as: J Immunol. 2019 Aug 9;203(6):1521–1531. doi: 10.4049/jimmunol.1801616

Figure 9. Pro-inflammatory genes’ mRNA expression is impaired in Y829F PARP1-expressing cells.

Figure 9.

Endogenous PARP1 in Raw 264.7 cells was silenced using siRNA targeting a distinguished sequence of PARP1, and human WT PARP1, Y829F PARP1 and Y907F PARP1 expressional plasmids were transfected and then the cells were stimulated with LPS or not for 1 h. Immunoblotting was performed to detect the interference of endogenous PARP1, as well as the ectopic expression of Flag-tagged WT, Y829F and Y907F PARP1 (a). Raw 264.7 cells as describe above were used, pro-inflammatory genes mRNA levels were detected by RT-PCR and electrophoresis (upper), or real-time PCR (lower) (b). Data were expressed as mean ± SD. Difference significance was analyzed by one-way ANOVA. **P < 0.01.