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. Author manuscript; available in PMC: 2019 Aug 8.
Published in final edited form as: Gene X. 2019 Feb 12;2:100007. doi: 10.1016/j.gene.2019.100007

Fig. 2.

Fig. 2.

Repression of p27 by Cux1 is relieved by HDAC inhibitors. 293 T cells were transiently transfected with 1 μg of reporter construct containing p27 upstream sequences from −1609 to +178 fused to the luciferase reporter gene (+), together with different concentrations of the Cux1 and Grg4 expression vectors (amounts shown are in μg) or with empty pcDNA3.1 expression vector (amounts shown are in μg). While increasing concentrations of Cux1 and Grg4 enhance repression of p27, treatment with 1.0 mg/ml of the HDAC inhibitor trichostatin A (TSA) relieves the repression of p27 promoter activity. Activity is expressed as the mean of three separate experiments performed in triplicate. Error bars indicate standard deviation. For statistical analysis, one-way ANOVA and Tukey post hoc analyses were used (*P < 0.05, **P < 0.01).