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. Author manuscript; available in PMC: 2020 Mar 31.
Published in final edited form as: Mucosal Immunol. 2019 Oct 9;13(1):64–74. doi: 10.1038/s41385-019-0206-9

Figure 4. Il-22ra2−/− mice have decreased pulmonary injury and increased tight junctions during influenza infection.

Figure 4.

BAL fluid was collected and analyzed from influenza-infected mice 5 days after infection. A) Cell cytotoxicity was detected by measuring lactate dehydrogenase. B) Total protein was measured using the BCA protein assay kit. C) Evan’s blue dye (0.1% in sterile PBS) was administered i.v. 30 minutes prior to collecting BAL. Evan’s blue is measured by absorbance at 640 on a spectrophotometer. D-F) bronchial brushings were performed to collect epithelial cells and RNA was purified and quantitative RT-PCR was performed for D) Tjp1, Tjp2, Cldn4 and Ocln. Data is presented as gene expression over uninfected controls. Statistical analysis was performed using one way ANOVA with Tukey’s post-hoc test(A,B) or Students T test(C,D) . *p<0.05, **p<0.01 **** p<0.0001.