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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Neuropsychopharmacology. 2009 Dec 2;35(3):806–817. doi: 10.1038/npp.2009.189

Figure 2. Fluorescence imaging and quantification of quinpirole-induced D2 receptor internalization.

Figure 2

A1. Surface D2 receptors were visualized with Zenon anti-FLAG Fab fragments directed to the FLAG epitope on the extracellular N-terminus (digitized to green) and the EYFP fluorescence of the EYFP tag on the D2 receptor C-terminus (digitized to red) in control cells (Q−). The overlap in the labeling appears yellow in the color merge shown. A2. Following quinpirole treatment (Q+), internalized D2 receptors show solely EYFP fluorescence (red). B. Quinpirole-induced D2 receptor internalization was quantitated using flow cytometry. B1. The histogram shows the distribution of fluorescence intensity for surface D2 receptors before (black) and after (red) quinpirole treatment. B2. Time course studies (n = 3; time constant as indicated) revealed that after quinpirole treatment, 42% D2 receptors remained on the surface, implying that ~58% underwent internalization.