Skip to main content
. Author manuscript; available in PMC: 2020 Mar 23.
Published in final edited form as: Mamm Genome. 2020 Jan 7;31(1-2):2–16. doi: 10.1007/s00335-019-09824-1

Fig 2. Cypridina luciferase reporter (Luc) assay in HEK293T cells.

Fig 2.

Cells were transfected with the control TK-luc plasmid or Stab2 promoter driven Luc reporter constructs with or without the 5’LTR sequence (arrows). Activities of luciferase in the medium were measured at 24 h after transfection. Signal intensities are normalized by a Gaussia luciferase. Data are shown relative to TK-Luc intensity as 1.0. (A) Luciferase activity of a 700 bp fragment upstream of the Stab2 gene in 129S6 and C57BL/6J (#1), of a 220 bp fragment as in the Stab2 gene of DBA/2J (#3), and with a 5’LTR in the reverse orientation (#6). Experiments were carried out with at least 4 wells per construct in each experiment and the mean ± SE of average activities from four experiments are presented. (B) Promoter activity assessed with the 5’LTR sequence of the Stab-IAP inserted upstream of the promoterless Luc in both orientations (#12 and #13), and general promoter activity assessed by insertion 3’ to the promoterless Luc in both orientations (#8 and #9). Data are the mean ± SE of average activities from three experiments.