Fig. 6.

Characterization of MfR using homogeneous model reaction. (A) Raw trace of Glu–GOx $R_{{\text{O}}_2}$ assay. Gray and black arrows show buffer blank and 4.85 μg mL⁻¹ GOx + 75 mM Glu in 50 mM KPi (pH 7.5), respectively. (B) O₂ consumption traces by select GOx concentrations. Solid lines indicate overlaid and averaged blank and reaction trials. Dashed lines represent ±SD (N = 5–6). (C) Dynamic range of the MfR using Glu–GOx $R_{{\text{O}}_2}$ expressed as mean ± SD, N = 3–5. (D) Inter-MfR reproducibility using Glu–GOx expressed as mean ± SD, N = 6–7.