Fig. 9.

Reversible inhibition of respiration in the MfR. (A) Trace of reversible respiratory inhibition by KCN in adherent ARPE-19 cells cultured on MfR. Arrows – buffer flow, arrowheads – static buffer, black – RB_C, gray – IB_c, white arrowhead – buffer exchange artifact. (B) $R_{{\text{O}}_2}$ quantitation of the results shown in (A). Black circles – respiration buffer, gray circles – inhibition buffer. (C) Classical states of whole cell $R_{{\text{O}}_2}$ in the adherent compared to suspension MfR configuration. Respiration was in glucose, lactate, pyruvate (10, 10, 1 mM), leak was by addition of oligomycin (2.5 μM), and uncoupled $R_{{\text{O}}_2}$ was by addition of CCCP (5 μM). Squares – adherent cells, gray faded circles – suspension (from Fig. 5). $R_{{\text{O}}_2}$ were normalized to uncoupled respiration and expressed as mean ± SD (N = 3).