Fig. 2.

DAT and NET-mediated uptake in midbrain (MB) and locus coeruleus (LC) cultures. a DA neurons in the midbrain culture express DAT (red) and NE cultures in the LC cultures expressed dopamine −β hydroxylase (DBH, green). b ³H-DA uptake was measured in LC and MB cultures. DA uptake in the midbrain cultures was sensitive to the DAT inhibitor, GBR12909, indicating predominantly DAT-mediated activity in those cultures. LC cultures, however, were unaffected by GBR12909 and instead sensitive to the NET-selective inhibitor desipramine (**p < 0.01 and ***p < 0.001 by one-way ANOVA compared to vehicle control)