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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Neurochem Res. 2020 Jan 7;45(6):1410–1419. doi: 10.1007/s11064-019-02939-6

Fig. 3.

Fig. 3

AMPH-mediated DAT and NET internalization in primary cultures is mediated by the small GTPase RhoA In MB cultures, DAT-mediated uptake (with the GBR12909 defined background already subtracted), a 30-min pre-treatment with AMPH (10 μM), leads to a dramatic loss of DAT transport capacity. However, this sensitivity to AMPH is abolished by pre-application of the RhoA Inhibitor I indicating a process mediated by the small GTPase RhoA. Similarly, NET-mediated, desipramine-sensitive 3H-DA transport in LC cultures was also sensitive to the AMPH pretreatment and similarly this loss of function was abolished by the RhoA inhibitor (*p < 0.05 by two-way ANOVA compared to vehicle control. F(3, 33) = 3.295)