Fig 1:

Evaluation of effects of CRISPR-RICTOR-Liposome on M2 polarized macrophages in vitro. (a) Immunoblotting analysis of RICTOR protein expression in untreated (MΦ) versus macrophages treated with CRISPR-RICTOR-Liposome (MΦ CRISPR). Isolated mouse macrophages were cultured, treated and analyzed by Western blot. (b) Densitometric analysis of RICTOR band intensities normalized to β-Actin. n=3, *significant to untreated macrophage control (MΦ) (p<0.05). (c) Effect on macrophage differentiation of CRISPR treatment targeting RICTOR, coupled with macrophage differentiation stimulated towards M1 (IFNγ+LPS) or M2 (IL-4+M-CSF). Cells were stained with CD163 (green-M2 marker) and CD80 (red-M1 marker). (d) mRNA expression from M2 macrophages with and without treatment with CRISPR-RICTOR-Liposome, measured via qPCR (n=4). (e) Quantitative analysis of cell phenotype. Scale bar = 100 μm, mean±SD, biological replicates n=5, *p<0.05, **p<0.01 vs. control.