Fig 2.

Viability of breast cancer cells growing in tumor spheres representing hypo-vascularized BCLM in 3D co-culture with M1 and M2 polarized macrophages. M1 macrophages were CRISPR-RICTOR-liposome treated and differentiated in the presence of IFN-gamma/LPS, while M2 macrophages were polarized in vitro in the presence of IL-4/M-CSF. The viability is shown as a function of varying ratios of M1:M2 macrophages under conditions of no treatment or exposure to MSV-nab-PTX for (A) 48h and (B) 72h. The varying ratios mimic the varying strength of a hypothetical polarization regimen that shifts this ratio. Mean±SD, biological replicates n=5, *p<0.05, **p<0.01 vs control.