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. Author manuscript; available in PMC: 2010 Mar 23.
Published in final edited form as: J Neurosci. 2009 Sep 23;29(38):11954–11964. doi: 10.1523/JNEUROSCI.2643-09.2009

Figure 7.

Figure 7

Neurons in the MnPO that project to the RMR do not project simultaneously to the DMH/DHA. Photomicrographs A-C illustrate the median preoptic nucleus after injection of CTb into the RMR (red in A) and Fluorogold into the DMH/DHA (blue in B). The merged image in C shows only one cell in this field that was doubly lableed (at the arrowhead). Panels D-F show the DMH/DHA in the same experiment. Note that the retrogradely labeled neurons that project to the RMR (red in D), are immediately adjacent to the tracer deposit at the core of the injection of Fluorogold into the DMH (marked INJ in panels E and F). The brightness of the Fluorogold image has been reduced in this set of photographs to avoid oversaturating the images; tracer spreads in all directions at least 500μm from the center of the injection as shown in fig. 6B, experiment RP20. Scale bar= 100μm. 3V: third ventricle; INJ: Fluorogold injection site; DMH/DHA: dorsomedial hypothalamic nucleus/dorsal hypothalamic area; MnPO: median preoptic nucleus; OVLT: organum vasculosum of the lamina terminalis.