Figure 3. Change in the reversal potential of glycinergic PSPs.

(A) Glycinergic/GABAergic PSPs were evoked at 0.5 Hz in a cell exhibiting spontaneous bursts of simple spikes. Four segments from a 150-sec-long V_m recording are shown in sequence. Complex spiking was induced after 20 sec of control period by 75 pA injection (depol) for 30 sec. The PSPs were depolarizing at V_m −77 mV during the pre-depolarization period. Just after the depolarization and complex spiking, PSPs were hyperpolarizing even at −81 mV but became depolarizing at −77 mV again over the next 100 sec. Recorded in the presence of 100 μM APV and 10 μM DNQX. (B) Evoked glycinergic/GABAergic PSPs shifting negatively after complex spiking. The bias current during the pre-depolarization period for cell 1, 2 and 3 was −45, −60 and 0 pA, respectively, and the current was adjusted post-depolarization to hold the V_m close to the pre-depolarization level.