FIGURE 4.

Individual animals display common hallmarks of running behavior. (a) Normalized position for 2 female (red) and 2 male (blue) examples. Normalized position results from the cumulative sum of the distance the animal ran over the habituation period divided by the final position of the animal. Running in the forward direction results in an end position at +1 while running in the reverse direction results in an end position at −1. (b) Acceleration plots for females and males for the last three days of habituation (day 5–7). Black arrows indicate active running while gray arrows indicate periods where the mouse is stationary. (c) Change plots displaying the change in direction between days. Columns represent the difference between the day listed and the day before. Rows are individual animals. The white boxes show a change in running direction from reverse to forward running. The black boxes are no change in direction. The gray boxes are a change from running forward to running in reverse (no gray boxes present)