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. Author manuscript; available in PMC: 2020 Jun 29.
Published in final edited form as: Glia. 2020 Feb 10;68(7):1479–1494. doi: 10.1002/glia.23796

FIGURE 3.

FIGURE 3

PDAC induces inflammatory mediator expression in vitro and in vivo. (a) Schematic representation of ex vivo KPC-conditioned media treatment system. (b) qRT-PCR analysis of pro-inflammatory, anti-inflammatory, and microglia-associated transcript from primary microglia treated with either KPC-conditioned media or 10 ng LPS. Values are relative to those from control media-treated primary microglia. All comparisons at least p < .01 compared to control media-treated in repeated measures one-way ANOVA unless indicated otherwise. n.s. = not significant. n = 3/group. Results are representative of two independent experiments. (c) qRT-PCR analysis of the same transcripts as B (except Tgfb) in RNA extracted from whole hypothalami. Results are relative to sham. ***p < .001, *p < .05 relative to sham comparing ΔCt values in repeated measures one-way ANOVA. Results consist of two independent experiments combined. n = 4–8/group