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. Author manuscript; available in PMC: 2020 Oct 12.
Published in final edited form as: Cancer Immunol Immunother. 2019 Oct 12;68(10):1661–1669. doi: 10.1007/s00262-019-02394-w

Figure 4: IDO activity is associated with decreased vaccine antigen-specific T-cell function.

Figure 4:

Peripheral blood mononuclear cells (PBMC) were obtained from patients treated with vaccine with or without pembrolizumab (after 12 weeks of treatment). PBMC were cultured in the presence of an overlapping peptide library derived from the PAP vaccine antigen (PAP), media alone, or phytohemaglutinin (PHA) as a positive control. Cells were also cultured with antigen in the presence of 1-MT. IFNγ-secreting T cells were detected by ELISPOT. Shown are representative ELISPOTS from two subjects (panel A), and cumulative data from 22 subjects evaluated (panel B). Statistical comparisons were made with a two-sided paired t test.