Fig 2.

DHM directly reduces ethanol-mediated mature SREBP-1 expression and lipid uptake in ethanol oxidizing (VL-17A) and non-oxidizing (HepG2) cell lines. A) Representative WB image of HepG2 and VL-17A cells cultured in EtOH and treated with 5 μM DHM or untreated for 24 hours and immunoblotted with anti-SREBP1 mAb (See Data S2A for ImageJ quantification of triplicates). B) HepG2 and VL-17A cells were cultured in 50 or 100 mM EtOH + 4 mM free fatty acids (2:1 Oleic to Palmitic acid) and either untreated or treated with either 2.5 μM or 5 μM DHM for 72 hours before photometric detection of intracellular lipid accumulation. Data represented as mean ± SEM. *p < 0.05 and p** < 0.01 compared with ethanol controls. †, p<0.05 vs. untreated control; n=3. A.U. = arbitrary units.