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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: JCO Precis Oncol. 2020 Mar 5;4:152–160. doi: 10.1200/PO.19.00294

Figure 4. Immune signaling activation in P2RY8-CRLF2 fusion AML.

Figure 4.

(A) Immunohistochemical photomicrographs of the bone marrow biopsy showing phospho-specific staining of STAT5 and ERK, but not STAT3. (B) Immunohistochemical photomicrographs of the bone marrow biopsy showing staining of PD-1 in scattered lymphoid cells. PD-L1 staining is equivocal, while PD-L2 is negative. (C) Using multiplex immunofluorescence microscopy, with digital image analysis, we find that the patient’s bone marrow is highly enriched with CD8+GZMB+ T-cells. CD34+ staining highlights the myeloblast population at the time of AML transformation. We further identify a population of CD3+CD4+ T-cells at baseline.