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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: J Neurosci. 2009 Oct 7;29(40):12702–12710. doi: 10.1523/JNEUROSCI.1166-09.2009

Fig. 2.

Fig. 2

Stimulation of CREB-dependent transcription by forskolin requires an increase in [Ca2+] within a microdomain. One day after transfecting with 4xCRE reporter plasmid, cultures were preincubated with Bapta-AM or EGTA-AM (10 μM) for 1 hr and then stimulated by addition of 10 μM forskolin. Cells were harvested 5 hr later for luciferase assays. Bapta completely inhibited forskolin-induced 4xCRE expression, whereas EGTA only inhibited by about 30%. ***, significantly different from forskolin+Bapta-AM (p<0.001); ns, not significantly different from untreated.