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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: J Neurosci. 2009 Oct 7;29(40):12702–12710. doi: 10.1523/JNEUROSCI.1166-09.2009

Fig. 4.

Fig. 4

Phosphorylation of CREB at serine-133 is not sufficient for 4xCRE transcription. (a) Immunoblots were probed for phospho-CREB (top) and then stripped and re-probed for total CREB (bottom) to control for sample variability. Forskolin (F, 10 μM) stimulated CREB phosphorylation when compared with untreated (U) cells. CREB phosphorylation induced by forskolin was insensitive to addition of ifenprodil (F+I) or Bapta-AM (F+B). The effects of forskolin were abolished by incubation with 10 μM of the PKA inhibitor, H89 (data not shown). (b) Quantification of CREB phosphorylation (P-CREB/CREB) from 4 experiments.