Fig. 9.

cAMP induces aspartate release from cortical neurons. Four hours after medium replacement, cultures were treated with 100 μM forskolin or vehicle (DMSO) for 30 min. 250 μl of the combined medium from duplicate cultures was removed and subjected to analysis by HPLC as described in Materials and Methods. (a) Chromatograms showing aspartate and glutamate peaks; forskolin induced an increase in medium aspartate without significantly affecting glutamate. (b) Summary of measurements from 10 pairs of cultures. Bars show means ± sem; ***, significantly different from control, p<0.005 by two-tailed t-test. ns, not significantly different from control.