Table 1.
Dilution | ||||
---|---|---|---|---|
Antibodies | Host | Vendor | In vivo | In vitro |
M1 | ||||
CD86 | Rat | BD Pharmingen, San Diego, CA | 1:100 | 1:100 |
iNOS | Rabbit | BD Pharmingen, San Diego, CA | N/A | 1:500 |
CD16/32 | Rat | BD Pharmingen, San Diego, CA | 1:800 | 1:1000 |
MHCII | Rat | AbD Serotec, Raleigh, NC | N/A | 1:100 |
M2 | ||||
Arginase I | Goat | Santa Cruz Biotechnology, Santa Cruz, CA | 1:200 | 1:200 |
CD206 | Goat | R&D Systems, Minneapolis, MN | 1:100 | 1:100 |
Microglia/macrophages | ||||
Tomato Lectin | Sigma-Aldrich, St Louis, MO | 1:1600 | N/A |