Table 2:
Primary Antibodies | Company | Dilution |
---|---|---|
Mouse monoclonal anti-GFP | Milipore MAB3580 |
1:200 |
Mouse monoclonal anti-actin | Calbiochem | 1:1000 |
Mouse Monoclonal anti-Zn5 | ZIRC; zfin.org/ZDB-ATB-081002-19 | 1:200 |
Mouse Monoclonal anti-Zpr1 (anti-Arrestin-3) | ZIRC; zfin.org/ZDB-ATB-081002-43 | 1:200 |
Mouse Monoclonal anti-Zpr-3 | ZIRC; zfin.org/ZDB-ATB-081002-45 | 1:200 |
Mouse anti-HPC1 | ZIRC; zfin.org/ZDB-ATB130225-1 | 1:200 |
Mouse anti-Zrf1 | ZIRC; zfin.org/ZDB-ATB-081002-46 | 1:1000 |
Mouse Monoclonal ZO-1A-12 | Innovative Research Cat# 33-9100, RRID:AB_87181 | 1:200 |
Mouse anti-BrdU | BD Biosciences Ca# 347580 | 1:100 |
Rat anti-BrdU | Abcam Cat# ab6326, RRID:AB_305426 | 1:200 |
Polyclonal anti-GNAT2 | MBL Ca# PM075 | 1:1000 |
Mouse Monoclonal anti-1D1 (ZF Rhodoposin) | Hyatt et al., 1996 Gift from Dr. Jim Fadool (FSU) |
1:1000 |
Mouse Monoclonal anti-4C4 (zebrafish microglia) |
Raymond et al., 2006
Hitchcock Lab |
1:200 |
Polyclonal anti-mCherry antibody | Abcam ab167453 | 1:200 |
Secondary Antibodies | ||
Alexa Fluor goat anti-mouse 488, 555, or 647 | Invitrogen | 1:500 |
Alexa Fluor goat anti-rabbit 488, 555, or 647 | Invitrogen | 1:500 |
Alexa Fluor goat anti-rat 488, 555, or 647 | Invitrogen | 1:500 |