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. Author manuscript; available in PMC: 2020 Jun 22.
Published in final edited form as: Arch Toxicol. 2020 Apr 1;94(6):1995–2007. doi: 10.1007/s00204-020-02728-z

Fig. 2.

Fig. 2

Picrotoxinin hydrolyses in plasma and has a short in vivo half-life. a LC/MS chromatograms of picrotoxinin after 0, 5, 20, 60, 90 and 180 min of incubation in mouse plasma. Picrotoxinin has a retention time of 3.19 min. The hydrolysis product has a retention time of 1.35 min. b Incubation of picrotoxinin at pH 9 produces the same hydrolysis product, while acidification can convert the hydrolysis product back into picrotoxinin. c Suggested structure of the hydrolysis product based on high-resolution mass spectrometry. d Total picrotoxinin plasma, liver and brain concentrations in mice; n = 6–9 mice per time point; shown mean ± SD values in nM