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. Author manuscript; available in PMC: 2020 Oct 23.
Published in final edited form as: STAR Protoc. 2020 Jun 4;1(1):100001. doi: 10.1016/j.xpro.2019.100001
DMEM/F-12
Knockout serum replacement 15% (v/v)
MEM-NEAA 1% (v/v)
Glutamax supplement 1% (v/v)
β-Mercaptoethanol 100 μM
LDN-193189 100 nM
SB431542 10 μM
Insulin 4 μg/mL

Store up to 10 days at 4°C.