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| DMEM/F-12* | |
|---|---|
| Neurobasalmedium* | |
| N2 supplement | 0.5% (v/v) |
| B27 supplement | 1% (v/v) |
| MEM-NEAA | 0.5% (v/v) |
| Glutamax supplement | 1% (v/v) |
| Penicillin/Streptomycin | 1% (v/v) |
| Insulin | 0.025% (v/v) |
| β-Mercaptoethanol | 50 μM |
| BDNF | 20 ng/m L |
| Ascorbic acid | 200 μM |
| Optional: FGF2 | 20 ng/mL (See Troubleshooting step 4) |
Store up to 2 weeks at 4°C.
DMEM/F-12 and neurobasal medium are mixed at 1:1 ratio.
