KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| TCF7L2 | Cell Signaling | Cat# 2569; RRID:AB_2199816 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| mTeSR1 | Stem Cell Technologies | Cat# 05875 |
| DMEM-F12 | Life Technologies | Cat# 11330057 |
| Neurobasal Media | Life Technologies | Cat# 2110349 |
| FBS | Life Technologies | Cat# 10437028 |
| Amino acids, non-essential | Life Technologies | Cat# 11140050 |
| Penicillin/Streptomycin | Life Technologies | Cat# 15140–122 |
| Glutamax | Life Technologies | Ca# 35050 |
| Insulin | Sigma | Ca# I9278 |
| β-Mercaptoethanol | Sigma | Ca# M7522 |
| N2 | Life Technologies | Cat# 17502–048 |
| B27 | Life Technologies | Cat# 17504–044 |
| B27 supplement without vitamin A | Life Technologies | Cat# 12587010 |
| bFGF | Millipore | Cat# GF003AF |
| KnockOut Serum Replacement | Life Technologies | Cat# 10828–028 |
| Matrigel | BD | Cat# 354230 |
| Y-27632 | Stem Cell Technologies | Cat# 72304 |
| Dispase (100 mL) | Stem Cell Technologies | Cat# 07913 |
| Accutase (100 mL) | Stem Cell Technologies | Cat# AT104 |
| LDN-193189 | Sigma | Cat# SML0559 |
| SB431542 | Abcam | Cat# ab120163 |
| XAV939 | Sigma | Cat# X3004 |
| BMP7 | GIBCO | Cat# PHC9544 |
| PD0325901 | Axon Medchem | Cat# Axon 1408 |
| BDNF | Prepotech | Cat# 450–02 |
| Ascorbic acid | Sigma | Cat# A92902 |
| Experimental Models: Cell Lines | ||
| HES-3 NKX2–1GFP/w | Elefanty lab | https://www.ncbi.nlm.nih.gov/pubmed/21425409 |
| Other | ||
| U-bottom ultra-low-attachment 96-well plate | Corning | CLS7007-24EA |
| Ultra-low-attachment 24-well plate | Corning | CLS3473 |
| Ultra-low-attachment 6-well plate | Corning | CLS3471-24EA |
| Orbital shaker | IKA | KS260 |